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IRCT20211103052956N3 IRCT 2026-10-18 Ragheb Isfahani higher education institute Resistance Training and Muscle Fiber Adaptation Effect of 12 Weeks of Progressive Resistance Training on Sex- and Fiber Type-Dependent Hypertrophy and Molecular Regulators in Young Adults 2026-10-12 anticipated 120 Recruiting https://irct.ir/trial/93807 interventional Randomization: Randomized, Blinding: Single blinded, Placebo: Not used, Assignment: Parallel, Purpose: Basic scienece, Other design features: This is a prospective, randomized, controlled, 2x2 factorial trial stratified by sex.Group (training vs control) and sex (men vs women) will between-subject factors. Time (pre vs post) and fiber type (Type I vs II) will be within-subject factors, Randomization description: Single blind, Blinding description: single blind. N/A Healthy skeletal muscle adaptation to resistance training. Intervention 1: Intervention group: Participants in the resistance training group will complete a supervised progressive resistance training program three times per week for 12 weeks with periodized loading. Weeks 1-4: 3 sets of 12 repetitions at 60% of one-repetition maximum. Weeks 5-8: 4 sets of 10 repetitions at 70% of one-repetition maximum. Weeks 9-12: 5 sets of 8 repetitions at 80% of one-repetition maximum. Exercise selection will include leg press, knee extension, leg curl, chest press, seated row, shoulder press, lat pulldown, biceps curl, triceps extension, and abdominal exercises. All training sessions will be supervised by certified exercise physiologists. Adherence exceede 95%. Intervention 2: Control group: Participants in the control group will receive no intervention during the 12-week study period and will instructed to maintain their usual physical activity and daily lifestyle habits. They will asked not to change their physical activity level during the study. Yes - There is a plan to make this available What will be shared: De-identified individual participant data that underlie the results reported in this article When: Beginning 12 months after publication of the article and ending 36 months after publication To whom: Researchers affiliated with academic or scientific institutions who provide a methodologically sound research proposal Conditions: Data will be shared for the purpose of reproducing the reported results or conducting secondary analyses. Requests must include a detailed research proposal, and a data sharing agreement must be signed. Data will be transferred via a secure electronic platform. The original research team must be acknowledged in any resulting publication. Where to obtain: Saeid Rostami, Raghob Isfahani Higher Education Institute, Isfahan, Iran. Email: srostami.20@gmail.com. Tel: 00989131088601. How to obtain: Interested researchers should submit a written request to the corresponding author via email, including a brief research proposal and the intended use of the data. The request will be reviewed by the principal investigator and the ethics committee within 4 weeks. If approved, a data sharing agreement will be signed, and de-identified data will be transferred via a secure platform within 2 weeks after signing the agreement. Comments: The data are not publicly available due to participant privacy. However, de-identified data may be available from the corresponding author upon reasonable request, subject to institutional and ethical approvals.
public Saeid Rostami
Isfahan
Isfahan Iran (Islamic Republic of) 1111111111 +98 31 4522 9250 srostami.20@gmail.com Ragheb Isfahani higher education institute scientific Saeid Rostami
Isfahan
Isfahan Iran (Islamic Republic of) 1111111111 +98 31 4522 9250 srostami.20@gmail.com Ragheb Isfahani higher education institute Iran (Islamic Republic of) Recreationally active adults aged 20-30 years BMI between 18.5 and 29.9 kg/m2 No structured resistance training in the prior 12 months 20 years 30 years Both Smoking Pregnancy Musculoskeletal disorders Supplement use Other Other Intervention group: Participants in the resistance training group will complete a supervised progressive resistance training program three times per week for 12 weeks with periodized loading. Weeks 1-4: 3 sets of 12 repetitions at 60% of one-repetition maximum. Weeks 5-8: 4 sets of 10 repetitions at 70% of one-repetition maximum. Weeks 9-12: 5 sets of 8 repetitions at 80% of one-repetition maximum. Exercise selection will include leg press, knee extension, leg curl, chest press, seated row, shoulder press, lat pulldown, biceps curl, triceps extension, and abdominal exercises. All training sessions will be supervised by certified exercise physiologists. Adherence exceede 95%. Control group: Participants in the control group will receive no intervention during the 12-week study period and will instructed to maintain their usual physical activity and daily lifestyle habits. They will asked not to change their physical activity level during the study. Cross-sectional area of Type I muscle fibers. Timepoint: Before the intervention (baseline) and 72 hours after the final training session. Method of measurement: Immunohistochemistry on transverse cryosections using myosin heavy chain isoform-specific antibodies, quantified with CellSens software. Ragheb Isfahani higher education institute Approved 2026-08-01 Ethics Committee of Islamic Azad University, Isfahan Branch Bassij Isfahan Isfehan Iran (Islamic Republic of)