IRCT20221128056644N1 IRCT 2023-06-03 Royan Institute The Efficacy of testicular PRP for patients with history of mTESE failure The Efficacy of Autologous Testicular Platelet Rich Plasma (PRP) for Patients with a History of Microdissection Testicular Sperm Extraction (mTESE) Failure: Clinical Trial 2024-02-20 anticipated 20 Complete https://irct.ir/trial/69854 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Treatment. 1 Non-obstructive azoospermia. Intervention group: Patients with non-obstructive azoospermia have at least one mTESE failure history. After receiving the permission of the ethics committee in the research, the research samples are selected based on the criteria for entering the study. After obtaining informed consent to participate in the research, they will be subjected to intratesticular injection of PRP. The PRP kit ( BD Vacutainer® Specialty Tubes, SKU:364606) will be used to prepare PRP. The duration of PRP preparation will be less than 45 minutes. Prepared PRP is injected into the spermatic cord or the interstitial space using a 5 mL syringe. In the third month after testicular PRP, gonadotropin stimulation in the patient's wife begins on days 2 to 4 of the menstrual cycle. Sperm extraction with the mTESE method will be performed at the same time as the egg retrieval day to use fresh sperm cells. In the first stage, 3 months after PRP injection into the testicles, sperm analysis will be checked in patients. The second stage will be 6 months after the injection. According to the obtained results, the sperm is injected into the cytoplasm of the egg by assisted reproductive techniques (ICSI) and fertilization takes place.. No - There is not a plan to make this available Justification or reason for not sharing IPD is There is no further information