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IRCT20221101056372N1 IRCT 2022-12-24 Tehran University of Medical Sciences Antibacterial efficacy of non-thermal atmospheric pressure plasma on dental caries Antibacterial efficacy of non-thermal atmospheric pressure plasma against Streptococcus mutans and lactobacillus acidophilus of carious dentin: a clinical study 2022-12-10 anticipated 10 Complete https://irct.ir/trial/66827 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Treatment. N/A dental caries. The studied population is people who have referred to the restorative department of Tehran Dental School for teeth restoration. After explaining the procedure to the patient and obtaining written consent from them, the patients are tested. The teeth that have cavities and clinical symptoms of spontaneous pain, irreversible pulpitis and deep decay are selected for study. The caries of the surface area (baseline samples) is removed with the help of a small size excavator, 2 mm long, 1 mm wide and 0.5 mm deep, equal to the volume of the excavator, and placed in an Ependorf tube containing a reduced transition medium based on thioglycollate without glucose. Then cold helium plasma is applied to the decay cavity by a plasma device containing helium gas (purity 99.999%) with a nozzle diameter of 3 mm, voltage of 10 kV, frequency of 6 kHz at a distance of 1 cm from the decay cavity for one minute and another sterile excavator of another sample is prepared in the vicinity and at the same level as the baseline sample, equal to the volume of the small size exciter, and is placed in an Ependorf tube containing a reduced transfer medium based on thioglycolate without glucose.Before treatment and after treatment, as much as possible, the patient's samples are collected in equal amounts in Ependorf tubes containing a reduced thioglycollate-based transfer medium without glucose. The samples are transferred to the microbiology research laboratory of Tehran University of Medical Sciences at ambient temperature within 24 hours. Mitis Salivarius agar containing valinomycin (MS-MUTV) and De Man Rogosa Sharpe agar containing maltose (MRSM) are used as selective and differential media for tracking and counting (determination of CFU/mL) S. mutans and L. acidophilus, respectively. to be After inoculation, the media are incubated for 48 hours in capnophilic conditions created by Gazpak in jars at 37°C. Then the CFU/mL of the studied bacteria will be calculated. Finally, the obtained numbers will be subjected to statistical analysis. The relationship between plasma and its effect on the number of bacteria on the tested samples is investigated. The significance level in this study is considered 0.05 (P value = 0.05).. Yes - There is a plan to make this available What will be shared: Raw data and analysis of bacterial counts are available for publication. Other data cannot be published.e When: access to data from 2024 To whom: for researchers working in academic institutions Conditions: For researchers, the CFU data of bacteria samples and analysis will be available. Where to obtain: mandana karimi Mandanakarimi1374@yahoo.com How to obtain: request to the researcher of thesis and determine clearly the purpose Comments:
public mandana karimi
no 10, 34th street, Gisha
tehran Iran (Islamic Republic of) 1448985464 +98 21 8611 2910 Mandanakarimi1374@yahoo.com Tehran University of Medical Sciences scientific mandana karimi
no 10, 34th street, Gisha
tehran Iran (Islamic Republic of) 1448985464 +98 21 8611 2910 mandanakarimi1374@yahoo.com Tehran University of Medical Sciences Iran (Islamic Republic of) dental cavitaed caries 18 years no limit Both systemic diseases endodontic dental pain Treatment - Other The studied population is people who have referred to the restorative department of Tehran Dental School for teeth restoration. After explaining the procedure to the patient and obtaining written consent from them, the patients are tested. The teeth that have cavities and clinical symptoms of spontaneous pain, irreversible pulpitis and deep decay are selected for study. The caries of the surface area (baseline samples) is removed with the help of a small size excavator, 2 mm long, 1 mm wide and 0.5 mm deep, equal to the volume of the excavator, and placed in an Ependorf tube containing a reduced transition medium based on thioglycollate without glucose. Then cold helium plasma is applied to the decay cavity by a plasma device containing helium gas (purity 99.999%) with a nozzle diameter of 3 mm, voltage of 10 kV, frequency of 6 kHz at a distance of 1 cm from the decay cavity for one minute and another sterile excavator of another sample is prepared in the vicinity and at the same level as the baseline sample, equal to the volume of the small size exciter, and is placed in an Ependorf tube containing a reduced transfer medium based on thioglycolate without glucose.Before treatment and after treatment, as much as possible, the patient's samples are collected in equal amounts in Ependorf tubes containing a reduced thioglycollate-based transfer medium without glucose. The samples are transferred to the microbiology research laboratory of Tehran University of Medical Sciences at ambient temperature within 24 hours. Mitis Salivarius agar containing valinomycin (MS-MUTV) and De Man Rogosa Sharpe agar containing maltose (MRSM) are used as selective and differential media for tracking and counting (determination of CFU/mL) S. mutans and L. acidophilus, respectively. to be After inoculation, the media are incubated for 48 hours in capnophilic conditions created by Gazpak in jars at 37°C. Then the CFU/mL of the studied bacteria will be calculated. Finally, the obtained numbers will be subjected to statistical analysis. The relationship between plasma and its effect on the number of bacteria on the tested samples is investigated. The significance level in this study is considered 0.05 (P value = 0.05). The CFU of dental caries bacteria. Timepoint: before and after. Method of measurement: Mitis Salivarius agar containing valinomycin and De Man Rogosa Sharpe agar containing maltose are used as selective and differential media for the detection and counting (determination of CFU/mL) of S. mutans and L. acidophilus, respectively. Tehran University of Medical Sciences Approved 2022-10-30 dentistry school, Tehran university of medical science no 10, 34th street, Gisha Tehran Tehran Iran (Islamic Republic of)