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IRCT20220510054809N1 IRCT 2022-06-27 Razi University of Kermanshah Effect of Aerobic Training with Vitamin D Supplementation on Gene Expression of Visceral Adipose Tissue of Obese Rats with Type 2 Diabetes Effect of Eight Weeks Aerobic Training with Vitamin D Supplementation on AMPK, PGC-1a, PTP1B, and UCP-1 Gene Expression in The Visceral Adipose Tissue of Obese Rats with Type 2 Diabetes 2022-07-21 anticipated 90 Complete https://irct.ir/trial/63633 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Basic scienece, Other design features: In this study, there are three training, supplementation, and training with supplementation groups in diabetic models; three training, supplementation, and training with supplementation groups in non-diabetic obese models; a diabetic group that receives a placebo; a non-diabetic obese group that receives a placebo; and a sham group, Randomization description: At first, any qualified rat got a specific code. then, the codes was written on a similar piece of paper and placed in the first vase. Also, the name of groups was written on a similar piece of paper and placed in the second vase. In the following, after blending the papers in any vase, a code were drawn from first vase and assigned to the group that its name were drawn from the second vase, respectively. In case of completing the desired members of any group before ending the lottery, the name of that group was excluded from its vase. N/A type 2 diabetes. Intervention 1: Intervention group 1; Aerobic training (type 2 diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks. Intervention 2: Intervention group 2; Aerobic training + Vitamin D supplementation (type 2 diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks; 5000 IU. Intervention 3: Intervention group 3; Vitamin D supplementation (type 2 diabetes): 5000 IU vitamin D supplementation per week for 8 weeks. Intervention 4: Intervention group 4; Aerobic training (obese without diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks. Intervention 5: Intervention group 5; Aerobic training + Vitamin D supplementation (obese without diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks; 5000 IU. Intervention 6: Intervention group 6; Vitamin D supplementation (obese without diabetes): 5000 IU vitamin D supplementation per week for 8 weeks. Intervention 7: Control group 1; Control (type 2 diabetes): 8 weeks of receiving placebo, without aerobic exercise. Intervention 8: Control group 2; Control (obese without diabetes): 8 weeks of receiving placebo, without aerobic exercise. Intervention 9: Sham group: Being on treadmill without doing exercise. Yes - There is a plan to make this available What will be shared: All the data are presentable. When: The data can be accessed 9 months after their publication. To whom: Only researchers of academic and scientific institutes can access the data. Conditions: All the data are usable with consideration of the authors rights. Where to obtain: 1- Dr. Rastegar Hosseini (rastegar.hoseini@gmail.com). 2- kimya khaledi (kimyakhaledi19@gmail.com). How to obtain: For this purpose one can send their request via e-mail to the aforementioned persons; after reviewing the request, in case of discretion, the related data will be presented to them. This process can take long between 3 to 10 days. Comments:
public Rastegar Hosseini
No. 9, Physical Education and Sports Science College, Razi University, Razi Blvd, Taq-e Bostan Ave
kermanshah Iran (Islamic Republic of) 6714967346 +98 83 3427 7605 R.hoseini@razi.ac.ir Razi University of Kermanshah scientific Rastegar Hosseini
No. 9, Physical Education and Sports Science College, Razi University, Razi Blvd, Taq-e Bostan Ave
Kermanshah Iran (Islamic Republic of) 6714967346 0098 34279265 R.hoseini@razi.ac.ir Razi University of Kermanshah Iran (Islamic Republic of) Age between8 to 9 months old Male sex To have type 2 diabetes in diabetic groups To have a minimum of 300 gram of weight No to have type 2 diabetes in non-diabetic obese groups 8 months 9 months Male Movement disabilities E11 Type 2 diabetes Treatment - Other Treatment - Other Treatment - Other Treatment - Other Treatment - Other Treatment - Other Placebo Placebo Other Intervention group 1; Aerobic training (type 2 diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks Intervention group 2; Aerobic training + Vitamin D supplementation (type 2 diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks; 5000 IU Intervention group 3; Vitamin D supplementation (type 2 diabetes): 5000 IU vitamin D supplementation per week for 8 weeks. Intervention group 4; Aerobic training (obese without diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks. Intervention group 5; Aerobic training + Vitamin D supplementation (obese without diabetes): Aerobic exercise between 25-60 minute with 50-60% of maximum oxygen consumption, 5 times per week for 8 weeks; 5000 IU Intervention group 6; Vitamin D supplementation (obese without diabetes): 5000 IU vitamin D supplementation per week for 8 weeks Control group 1; Control (type 2 diabetes): 8 weeks of receiving placebo, without aerobic exercise Control group 2; Control (obese without diabetes): 8 weeks of receiving placebo, without aerobic exercise Sham group: Being on treadmill without doing exercise. AMPK Gene Expression. Timepoint: Before intervention; after intervention. Method of measurement: Western-Blot method using Kiagen Kit With 0/021 Pg/mL sensitivity. Expression Of PGC-1a Gene. Timepoint: Before intervention; after intervention. Method of measurement: Real time-PCR Method using Mirnasy mini kit50 Kit With 0/06 ng/mL sensitivity. Expression Of PTP1B Gene. Timepoint: Before intervention; after intervention. Method of measurement: Real time-PCR Method using RNeasy Commercial Kit With 0/06 ng/mL sensitivity. Expression Of UCP-1 Gene. Timepoint: Before intervention; after intervention. Method of measurement: Eliza Double Sandwich Method using Cat no: ZB-11252-R9648 Kit With 0/06 ng/mL sensitivity. Insulin Resistance Index. Timepoint: Before intervention; after intervention. Method of measurement: Serum Insulin by Elisa Method using Micro-calorie Metric Kit With 5/5 mg/dl Sensitivity. Insulin Resistance Index will be Determined Using Glucose And Insulin Concentrations And HOMA-IR formula.HOMA-IR= (Glucose Concentration × Insulin Concentration)÷22/5. Irisin Expression Gene. Timepoint: Before intervention; after intervention. Method of measurement: Eliza Method using EASTBIOPHARM Kit With 0/023 mg/mL sensitivity. Razi University of Kermanshah Approved 2022-04-19 Ethics committee in research of Razi University of Kermanshah No. 9, Razi University, Daneshgah Blvd, Taq-e Bostan Ave Kermanshah Kermanshah Iran (Islamic Republic of)