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IRCT20201121049453N1 IRCT 2021-01-27 University of Kurdistan The effect of Resistance Training with Creatine Monohydrate Supplementation on Oxidative Stress and Antioxidant Indices The Effects of 8 Weeks of Resistance Training with Creatine Monohydrate Supplementation on Oxidative Stress and Antioxidant Indices in Older Adults 2021-02-19 anticipated 45 Complete https://irct.ir/trial/52480 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Used, Assignment: Parallel, Purpose: Basic scienece, Randomization description: Simple randomization: First each subject will be taken in each groups (A or B) by tossing a coin. Each side of the coin determines the assignment of each subject. Then each group will be named as control or experimental group by tossing a coin, Blinding description: First, the subjects in the experimental group are randomly divided into two groups: resistance training + creatine monohydrate supplement and resistance training + placebo. The subjects will not know in which group they are. Creatine monohydrate and placebo were identical in amount, taste, texture, color, and appearance. The supplement packages were coded so that neither the investigators nor the participants were aware of the contents until completion of the analyses. The supplements were provided by a staff member of research team who did not have any participation in the data acquisition, analyses, and interpretation. N/A Resistance training. Intervention 1: Intervention group 1: 15 participants as a resistance training + creatine monohydrate supplement group received 0.1g•kg/day of creatine (under license from Canada, Gene Star Company and approved by the Ministry of Health of Iran) every day for 8 weeks. subjects consumed the supplement as a single dose after training session (similar time in non training dayes). The supplement packages were coded so that neither the investigators nor the participants were aware of the contents until completion of the analyses. The supplements were provided by a staff member of research team who did not have any participation in the data acquisition, analyses, and interpretation. The training protocol will consist of 3 sets of 10 reps at 75% of 1RM. Resting time between sets and movements were performed for 2 and 3 minutes , respectively. Blood sampling will be performed 48 hours before the start of training and 48 hours after the last training session in the fasting state. Intervention 2: Intervention group 2: 15 participants as a resistance training + placebo group did resistance training for 8 weeks. The training was consist of 3 sets of 10 reps at 75% of 1RM. Resting time between sets and movements were performed for 2 and 3 minutes , respectively. Creatine and placebo (Globe Plus 10 DE Maltodextrin, Univar Canada) were identical in amount, taste, texture, color, and appearance. Fasting blood sampling were taken 48 hours before the training and 48 hours after the last training session. Intervention 3: Control group: 15 participant as control group were asked not to participate in any exercise program and keep on their regular diet during research and report any changes in their life like disease, serious stress. Yes - There is a plan to make this available What will be shared: All Data When: 6 months after data publication To whom: Student and academic staff Conditions: every analyses is fine for future research Where to obtain: researcher How to obtain: By sending email to researcher Comments:
public Ehsan Amiri
Pasdaran boulevard
Sanandaj Iran (Islamic Republic of) ۶۶۱۷۷-۱۵۱۷۵ +98 87 3366 4600 ehsan.amiri93@gmail.com University of Kurdistan scientific Dariush Sheikholeslami-Vatani
Pasdaran boulevard
Sanandaj Iran (Islamic Republic of) ۶۶۱۷۷-۱۵۱۷۵ +98 87 3366 4600 d.vatani@uok.ac.ir University of Kurdistan Iran (Islamic Republic of) Age of 55-70 years old 55 years 70 years Both Back pain History of lumbar surgery Cardiovascular disease Metabolic sickness history Clinical limitations for sports activities Y93.B Activities involving other muscle strengthening exercises Other Placebo Other Intervention group 1: 15 participants as a resistance training + creatine monohydrate supplement group received 0.1g•kg/day of creatine (under license from Canada, Gene Star Company and approved by the Ministry of Health of Iran) every day for 8 weeks. subjects consumed the supplement as a single dose after training session (similar time in non training dayes). The supplement packages were coded so that neither the investigators nor the participants were aware of the contents until completion of the analyses. The supplements were provided by a staff member of research team who did not have any participation in the data acquisition, analyses, and interpretation. The training protocol will consist of 3 sets of 10 reps at 75% of 1RM. Resting time between sets and movements were performed for 2 and 3 minutes , respectively. Blood sampling will be performed 48 hours before the start of training and 48 hours after the last training session in the fasting state. Intervention group 2: 15 participants as a resistance training + placebo group did resistance training for 8 weeks. The training was consist of 3 sets of 10 reps at 75% of 1RM. Resting time between sets and movements were performed for 2 and 3 minutes , respectively. Creatine and placebo (Globe Plus 10 DE Maltodextrin, Univar Canada) were identical in amount, taste, texture, color, and appearance. Fasting blood sampling were taken 48 hours before the training and 48 hours after the last training session. Control group: 15 participant as control group were asked not to participate in any exercise program and keep on their regular diet during research and report any changes in their life like disease, serious stress Malondialdehyde (MDA). Timepoint: Before and after 8 weeks resistance training. Method of measurement: By ELISA method. Glutathione peroxidase (GPX). Timepoint: Before and after 8 weeks resistance training. Method of measurement: By ELISA method. Total antioxidant capacity (TAC). Timepoint: Before and after 8 weeks resistance training. Method of measurement: By ELISA method. 8-hydroxy-2' -deoxyguanosine (8-OHdG). Timepoint: Before and after 8 weeks resistance training. Method of measurement: By ELISA method. Muscle strength. Timepoint: Before and after 8 weeks resistance training. Method of measurement: One Repetition maximum. Blood Creatinine. Timepoint: Before and after 8 weeks resistance training. Method of measurement: Blood sampling. Quality of Life. Timepoint: Before and after 8 weeks resistance training. Method of measurement: Questionnaire (SF-36) Quality of Life. Body mass index. Timepoint: Before and after 8 weeks resistance training. Method of measurement: weight(kg)/height(m2). University of Kurdistan Approved 2020-06-06 Ethics commitee of Kurdistan University Pasdaran boulevard Sanandaj Kurdistan Iran (Islamic Republic of)