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IRCT20101115005181N16 IRCT 2020-06-12 Tehran University of Medical Sciences Study of the expression of genes involved in the process of death in oocytes in four groups of patients undergoing ovulation stimulation cycle with four therapies. Study of the expression of genes involved in the process of apoptosis in cellulose cells in four groups of patients under the cycle of ovulation stimulation with antagonist or agonist GNRH with HP-FSH or FSH recombinant. 2020-06-01 anticipated 80 Complete https://irct.ir/trial/48514 interventional Randomization: Not randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Diagnostic. 2-3 Female Infertility. Intervention 1: The first intervention group: 20 infertile patients who were stimulated using the Gnrh agonist ampoule (Cinafact) of Cinagen Company, from the 21th day of previous cycle , 20 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (HP) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. Intervention 2: The second intervention group: 20 infertile patients who were stimulated using the Gnrh agonist ampoule (Cinafact) of Cinagen Company, from the 21th day of previous cycle , 20 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (Recombinant) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. Intervention 3: The third intervention group: 20 infertile patients who were stimulated using the Gnrh antagonist ampoule (cetrotide) of Merck Company, from the 6th day of induction ovulation, 0.25 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (HP) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. Intervention 4: The Fourth intervention group: 20 infertile patients who were stimulated using the GnRH antagonist ampoule (cetrotide) of Merck Company, from the 6th day of induction ovulation, 0.25 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (recombinant) of Cinagen company from the third day of the menstrual cycle, are administrated for at least 10 days. In all intervention groups of 1-4; The eggs are aspirated from the follicular fluid and rinsed several times, then a drop of hyaluronidase is added to them and the cumulus is separated.Gene extract from Cumulus, At this stage, first RNA is extracted and then C DNA; It is synthesized and used; Gene expression evaluation is used using real-time PCR method. Undecided - It is not yet known if there will be a plan to make this available Justification or reason for indecision in sharing IPD is There is no further information.
public Saeed Azizollahi
IVF Labratoary, Vali- Asr Hospital, Blvd Keshavarz.
Tehran Iran (Islamic Republic of) 1419733141 +98 21 6658 1616 saeedazizollahi@yahoo.com Tehran University of Medical Sciences scientific Batool Hossein Rashidi
Vali-e-Asr Reproductive Health Research Center, Imam Hospital Complex, Keshavarz Blvd.
Tehran Iran (Islamic Republic of) 1419733141 +98 21 6658 1616 bhrashidi@yahoo.com Tehran University of Medical Sciences Iran (Islamic Republic of) The age of 20- 30 years old. Normal response to induction of ovulation. 20 years 30 years Female unexplained infertility Cause Azoospermia Ovarian hyper stimulation Syndrome N97.0 Female infertility associated with anovulation Treatment - Drugs Treatment - Drugs Treatment - Drugs Treatment - Drugs The first intervention group: 20 infertile patients who were stimulated using the Gnrh agonist ampoule (Cinafact) of Cinagen Company, from the 21th day of previous cycle , 20 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (HP) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. The second intervention group: 20 infertile patients who were stimulated using the Gnrh agonist ampoule (Cinafact) of Cinagen Company, from the 21th day of previous cycle , 20 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (Recombinant) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. The third intervention group: 20 infertile patients who were stimulated using the Gnrh antagonist ampoule (cetrotide) of Merck Company, from the 6th day of induction ovulation, 0.25 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (HP) of Cinagen company from the third day of menstrual cycle, are administrated for at least 10 days. The Fourth intervention group: 20 infertile patients who were stimulated using the GnRH antagonist ampoule (cetrotide) of Merck Company, from the 6th day of induction ovulation, 0.25 IU subcutaneous daily up to 36 hours before oocyte pick up and 225 units FSH (recombinant) of Cinagen company from the third day of the menstrual cycle, are administrated for at least 10 days. In all intervention groups of 1-4; The eggs are aspirated from the follicular fluid and rinsed several times, then a drop of hyaluronidase is added to them and the cumulus is separated.Gene extract from Cumulus, At this stage, first RNA is extracted and then C DNA; It is synthesized and used; Gene expression evaluation is used using real-time PCR method. Maturation of Oocyte. Timepoint: 36 hours after the last administration of treatment. Method of measurement: Electronic Microscope. Fertilization rate. Timepoint: 24 hours after intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope. Rate of Embryo development. Timepoint: The third day of intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope. The evaluation of Gene expression of HAS-2, BAX, BCL-2, VCAN, ALCAM, R PTGS-2 in cumulus of oocyte. Timepoint: The day of pick up ovum. Method of measurement: Real time PCR. Tehran University of Medical Sciences Approved 2017-06-02 Ethics committee of Tehran University of Medical Sciences Sixth floor, Deputy of Research and Technology, Central University Organization, Quds Street, , Keshavarz Boulevard, Tehran Tehran Iran (Islamic Republic of)