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IRCT20140707018381N4 IRCT 2018-08-03 Shahid Beheshti University of Medical Sciences Effects of calcium ionophore on human oocyte in vitro maturation outcome Effects of calcium ionophore on human oocyte in vitro maturation outcome, fertilization rate and embryo development 2017-10-23 anticipated 150 Complete https://irct.ir/trial/29169 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment, Randomization description: The sampling method will be two-stage sampling methods. In the first stage, non-probability sampling method is used and in the second stage, random allocation is performed using permuted block randomization between the two intervention and control groups. 3 Infertility. Intervention 1: Intervention group: Treatment group with calcium ionophore: Each oocyte is placed in a 30-ml droplet of ionophore calcium A23187 for 15 minutes, then washed in two drops of 30 μL of the culture medium. The oocytes are then culture in an ecobar for 24-28 hours. Intervention 2: Control group: Group without treatment with ionophore calcium. Yes - There is a plan to make this available What will be shared: All interventions in the patient's file will be recorded and archived in the Taleghani hospital. All data obtained from this study will be published with regard to the privacy and confidentiality right of the the information of research participants. When: Get started 6 months after publishing study results To whom: The findings of this study will be accessible to all individuals. Conditions: To improve pregnancy outcomes in infertility clinics Where to obtain: Elham Fazeli Email: Elhamfazeli15@yahoo.com Tel:00989112448530 How to obtain: Data that release in the published article will be available to other people according to the journal protocol that published article (free or purchase). In order to access data that not published in the paper, it is required to obtain the correspond author's approval and then approval of the research deputy of Shahid Beheshti University of Medical Sciences is obligatory. Comments:
public Elham Fazeli
Faculty of medicine, Arabi Ave, Daneshjo street, Velenjak, Tehran
Tehran Iran (Islamic Republic of) 1983969411 +98 13425965 Elhamfazeli15@yahoo.com Shahid Beheshti University of Medical Sciences scientific Elham Fazeli
Faculty of medicine, Arabi Ave, Daneshjo street, Velenjak, Tehran
Tehran Iran (Islamic Republic of) 1983969411 +98 13425965 Elhamfazeli15@yahoo.com Shahid Beheshti University of Medical Sciences Iran (Islamic Republic of) Approval of the Ethics Committee of Shahid Beheshti University of Medical Sciences The age of a woman is between 20-40 years old Sperm has a normal parameter based on WHO criteria 20 years 40 years Both Endometriosis Poly cystic ovary syndrome Genetic disorders N97.9 Female infertility, unspecified Treatment - Drugs Treatment - Drugs Intervention group: Treatment group with calcium ionophore: Each oocyte is placed in a 30-ml droplet of ionophore calcium A23187 for 15 minutes, then washed in two drops of 30 μL of the culture medium. The oocytes are then culture in an ecobar for 24-28 hours. Control group: Group without treatment with ionophore calcium Oocyte cytoplasmic maturation. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Cell Tracker Blue staining for oocyte cytoplasmic maturity. Oocyte oxidative stress. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Evaluation of the free radical oxygen (ROS) in cytoplasmic by staining with H2DCFDA. Meiotic spindle structure. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Evaluation of miieotic spindle structure by staining with anti-tubulin antibody. Apoptosis rate. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Determination of apoptosis rate by evaluating expression bax, bcl2 and caspase-3 gene by Real Time-PCR. Nucleation maturation. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Determining the oocyte nucleus maturity by evaluating expression of MPF and MAPK by Real Time-PCR. Fertilization rate. Timepoint: 16-18 hours after ICSI. Method of measurement: Divide the total number of oocytes fertilized to the total oocytes under ICSI. Cleavage rate. Timepoint: The third day after ICSI. Method of measurement: Split the total number of cleavage embryos divided into fertilized embryos. Embryo quility. Timepoint: The third day after ICSI. Method of measurement: Grading of embryo morphology based on the number and size of the blastomeres and the rate of fragmentation. Shahid Beheshti University of Medical Sciences Approved 2017-09-28 Ethics Committee of Shahid Beheshti University of Medical Sciences Faculty of medicine, Arabi Ave, Daneshjo street, Velenjak, Tehran Tehran Tehran Iran (Islamic Republic of)