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IRCT2017050833869N1 IRCT 2017-05-29 Shiraz University of Medical Sciences The Effect of 12 Weeks Aerobic, Resistance and Combined Exercises on Omentin-1 Levels and Insulin Resistance among Type 2 Diabetic Middle-Aged Women The Effect of 12 Weeks Aerobic, Resistance and Combined Exercises on Omentin-1 Levels and Insulin Resistance among Type 2 Diabetic Middle-Aged Women 2016-08-01 anticipated 60 Complete https://irct.ir/trial/26019 interventional Randomization: Randomized, Blinding: Single blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment. N/A Diabetes. Intervention 1: All volunteers were examined by a general physician to assure that the intervention would not endanger patients. All participants gave their written informed consent. Group assignment: patients were divided into four groups each consisting of 15 participants. A group (control group) received no intervention and three groups underwent ‘AEs,’ ‘REs,’ group, and ‘CEs’ groups. For those in AE group, each exercise session consisted of three phases of warm up, the main stage and a cool-down period. The warm-up phase comprised of 20 minutes of stretching and jogging. The main phase of the study was consisted of 25 minutes exercise in order to achieve 50% to 55% of maximum heart rate as measured by cycle ergo-meter. Running, exercise and stretching made up the cooling-down phase. Intervention 2: Each phase in the RE group had three sessions per week which consisted of three phases of warm-up, the main, and cooling-down. The warm-up involved 20 minutes of stretching exercises and jogging on the spot. The main phase consisted of three sets×eight repetitions of weight training including leg extension, prone leg curl, abdominal crunch, biceps, triceps, and seated calf. The exercise intensity was 50% to 55% of one repetition maximum or 1 repetition maximum (RM). The cooling-down also consisted of running, free exercises and stretching. Intervention 3: The group with CEs had the same schedule of other groups for warm-up and cooling-down. The main phase consisted of aerobic training integrated with RE, with half the execution time and the same intensity of resistance and aerobic groups. The trainings programs were performed within three sessions per week for 12 weeks. Every 2 weeks, in all exercise groups training was increased by 5 minutes and the intensity by 5%. The average intensity of main stage in every exercise group was 5.5 metabolic equivalent of task (MET) in first week and increased to 7.1 MET at the end of the study. Intervention 4: control group received no intervention.
public Mostafa Dianati
Shiraz University of Medical Sciences
Shiraz Iran (Islamic Republic of) +98 917 841 3762 dianatinasab@sums.ac.ir Shiraz University of Medical Sciences scientific Mohammad Fararouei
Shiraz University of Medical Sciences
Shiraz Iran (Islamic Republic of) +98 917 310 6583 fararooei@yahoo.com Shiraz University of Medical Sciences Iran (Islamic Republic of) The inclusion criteria were: having been suffering from T2DM (fasting blood sugar ≥126 mg/dl and 2-hour postprandial blood glucose ≥200 mg/dl) for at least 2 years; being female; aged 45 to 60 years exclusion criteria: diagnosed with any other diseases 40 years 50 years Female E11 Non-insulin-dependent diabetes mellitus Other Other Treatment - Other N/A All volunteers were examined by a general physician to assure that the intervention would not endanger patients. All participants gave their written informed consent. Group assignment: patients were divided into four groups each consisting of 15 participants. A group (control group) received no intervention and three groups underwent ‘AEs,’ ‘REs,’ group, and ‘CEs’ groups. For those in AE group, each exercise session consisted of three phases of warm up, the main stage and a cool-down period. The warm-up phase comprised of 20 minutes of stretching and jogging. The main phase of the study was consisted of 25 minutes exercise in order to achieve 50% to 55% of maximum heart rate as measured by cycle ergo-meter. Running, exercise and stretching made up the cooling-down phase. Each phase in the RE group had three sessions per week which consisted of three phases of warm-up, the main, and cooling-down. The warm-up involved 20 minutes of stretching exercises and jogging on the spot. The main phase consisted of three sets×eight repetitions of weight training including leg extension, prone leg curl, abdominal crunch, biceps, triceps, and seated calf. The exercise intensity was 50% to 55% of one repetition maximum or 1 repetition maximum (RM). The cooling-down also consisted of running, free exercises and stretching. The group with CEs had the same schedule of other groups for warm-up and cooling-down. The main phase consisted of aerobic training integrated with RE, with half the execution time and the same intensity of resistance and aerobic groups. The trainings programs were performed within three sessions per week for 12 weeks. Every 2 weeks, in all exercise groups training was increased by 5 minutes and the intensity by 5%. The average intensity of main stage in every exercise group was 5.5 metabolic equivalent of task (MET) in first week and increased to 7.1 MET at the end of the study. control group received no intervention Insulin Resistance. Timepoint: At 2 stage. First day of the study (before any intervention) and second mesure after 12 weeks intervention. Method of measurement: اندازه گیری با اسفاده از کیت انسولین و فرمول روبرو: Insulin resistance =[fasting glucose (mg/dL)×fasting insulin (mU/L)/405]. Before intervention, 5 mL blood samples were taken after 12 hours of fasting and the serum samples were separated using a centrifuge. The samples were frozen at –70° degrees centigrade until the time of serum analysis and omentin-1 and plasma glucose were then measured. Serum omentin-1 and fasting insulin concentrations were determined using enzyme-linked immunosorbent assay kits (Cristal day biotech, Shanghai, China) and (Monobind, Aachen, Germany). Fasting plasma glucose was measured using glucose kit (Pars Azmoon, Tehran, Iran) through the photometric method. Insulin resistance was determined through homeostasis model evaluation (HOMA-IR) and based on the following equation: HOMA-IR=[fasting glucose (mg/dL)×fasting insulin (mU/L)/405]. Timepoint: before intervention, 5 mL blood samples were taken after 12 hours of fasting and the serum samples were separated using a centrifuge. The samples were frozen at –70° degrees centigrade until the time of serum analysis and omentin-1 and plasma glucose were then measured. Serum omentin-1 and fasting insulin concentrations were determined using enzyme-linked immunosorbent assay kits (Cristal day biotech, Shanghai, China) and (Monobind, Aachen, Germany). Fasting plasma glucose was measured using glucose kit (Pars Azmoon, Tehran, Iran) through the photometric method. Insulin resistance was determined through homeostasis model evaluation (HOMA-IR) and based on the following equation: HOMA-IR=[fasting glucose (mg/dL)×fasting insulin (mU/L)/405]. Method of measurement: before intervention, 5 mL blood samples were taken after 12 hours of fasting and the serum samples were separated using a centrifuge. The samples were frozen at –70° degrees centigrade until the time of serum analysis and omentin-1 and plasma glucose were then measured. Serum omentin-1 and fasting insulin concentrations were determined using enzyme-linked immunosorbent assay kits (Cristal day biotech, Shanghai, China) and (Monobind, Aachen, Germany). Fasting plasma glucose was measured using glucose kit (Pars Azmoon, Tehran, Iran) through the photometric method. Insulin resistance was determined through homeostasis model evaluation (HOMA-IR) and based on the following equation: HOMA-IR=[fasting glucose (mg/dL)×fasting insulin (mU/L)/405]. Change in plasma omentin-1. Timepoint: before and after interventiond. Method of measurement: before intervention, 5 mL blood samples were taken after 12 hours of fasting and the serum samples were separated using a centrifuge. The samples were frozen at –70° degrees centigrade until the time of serum analysis and omentin-1 and plasma glucose were then measured. Serum omentin-1 and fasting insulin concentrations were determined using enzyme-linked immunosorbent assay kits (Cristal day biotech, Shanghai, China) and (Monobind, Aachen, Germany). Fasting plasma glucose was measured using glucose kit (Pars Azmoon, Tehran, Iran) through the photometric method. Insulin resistance was determined through homeostasis model evaluation (HOMA-IR) and based on the following equation: HOMA-IR=[fasting glucose (mg/dL)×fasting insulin (mU/L)/405]. Shiraz University of Medical Sciences Approved 2017-02-22 Shiraz University of Medical Sciences ethical comity Shiraz University of Medical Sciences Shiraz Iran (Islamic Republic of)