IRCT2016040627260N1 IRCT 2016-05-10 Vice-chancellor for Research of Bushehr University of Medical Sciences Efficacy of autologous plasma therapy on clinical symptoms of Chronic Spontaneous Urticaria Clinical Trial on efficacy of autologous plasma therapy on clinical symptoms of Patients with Chronic Spontaneous Urticaria Compared with no treatment 2016-04-08 anticipated 30 Complete https://irct.ir/trial/22373 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Treatment. N/A Chronic Spontaneous Urticaria. Patients will be divided into idiopathic and autoimmune forms of urticaria after the autologous plasma skin test. After consent to participate in the study, 5cc of brachial vein blood will be sampled first. Then we perform intradermal injection of equal measure from 0.1 cc of patient's centrifuged serum, histamine as a positive control and 0.9 % saline as a negative control at a distance of 3-5 cm from each other in the forearm and in the area that is free of rash in the past 48 hours with insulin syringes. Autologous plasma skin test is considered positive when after 30 minutes the swelling and redness diameter of serum injection area is equal or more than 1.5 mm greater than the swelling and redness of negative control injection site (normal saline). In order to treat with autologous plasma, we will take 5 ml of blood from the brachial vein using heparinized 10 ml sterile Falcon tubes from the subjects in Allergy Clinic in Bushehr Martyrs Hospital in Persian Gulf in every week for 10 weeks. Patients' plasma will be separated by centrifugation at 1000 rpm for 10 minutes and 2 cc of it will be injected deep intramuscular in gluteal or arms area alternatively..