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IRCT2014090819091N1 IRCT 2014-09-13 Babol university of medical sciences Radio protective effect of royal jelly against ionizing-radiation Determination of radio protective effect of Royal Jelly on ionizing radiation-induced DNA damage in human peripheral blood lymphocytes 2015-02-04 anticipated 30 Complete https://irct.ir/trial/17174 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Prevention. N/A Condition 1: Male infertility. Condition 2: Radiation exposure. Condition 3: Ionizing radiation exposure. This is an quasi-experimental study just including intervention group. In this study each participant gives blood samples twice. First sampling, on admission to the fertility center. After first sampling each participant would start taking orally 1000 mg royal jelly per day for two weeks. Second sampling would happen after this two-week period. In each sampling step, blood samples would be divided into two parts,pre-irradiated and post-irradiated, and all of them would be investigated regarding DNA damage by Comet Assay..
public Dr. Ali Shabestani Monfared
Babol university of medical sciences, Ganj afrooz Av, Babol, Mazandaran
Babol Iran (Islamic Republic of) +98 11 3528 9256 monfared_ali@yahoo.commonfared1345@gmail.com Babol university of medical sciences scientific Dr. Maryam Shahidi
Mazandaran university of medical sciences, Begining of Vali-Asr highway, Imam square, Sari,Mazandaran
Sari Iran (Islamic Republic of) +98 11 3334 3333 shahidi_maryam@yahoo.cominfo@ivfsari.com Mazandaran university of medical sciences Iran (Islamic Republic of) Inclusion criteria: men; at the age of 20 to 45 year old; Exclusion criteria: having a history of cancer; autoimmune diseases; having allergy to bee sting, to honey, to royal jelly and pollen; having a history of smoking; radiotherapy; chemotherapy; nuclear medicine scan; antibiotic therapy during last three months before attending the study. 20 years 45 years Male N46 Z58.4 W88 Male infertility Exposure to radiation Exposure to ionizing radiation Prevention This is an quasi-experimental study just including intervention group. In this study each participant gives blood samples twice. First sampling, on admission to the fertility center. After first sampling each participant would start taking orally 1000 mg royal jelly per day for two weeks. Second sampling would happen after this two-week period. In each sampling step, blood samples would be divided into two parts,pre-irradiated and post-irradiated, and all of them would be investigated regarding DNA damage by Comet Assay. Lymphocyte DNA tail length. Timepoint: A)On admission B)Two weeks after oral administration of royal jelly. Method of measurement: Visual scoring of DNA tail. Sperm count in semen sample. Timepoint: A)On admission B)75 days after oral administration of royal jelly. Method of measurement: Hemocytometer. Gamma radiation dose. Timepoint: A)On admission B)75 days after oral administration of royal jelly. Method of measurement: in Gy scale measured by gamma radiation dosimeter. Sperm morphology in semen sample. Timepoint: A)On admission B)75 days after oral administration of royal jelly. Method of measurement: Hemocytometer. Sperm motility in semen sample. Timepoint: A)On admission B)75 days after oral administration of royal jelly. Method of measurement: Hemocytometer. Hazrat-e Maryam Fertility Center Babol university of medical sciences Hazrat-e Maryam Fertility Center Approved 2014-08-16 Babol university of medical sciences Babol university of medical sciences, Ganj afrooz Av, Babol, Mazandaran Babol Iran (Islamic Republic of)