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IRCT2014081618814N1 IRCT 2014-12-26 Vice chancellor for research, Tehran University of Medical Sciences Effect of Omega-3 supplementation on immune responses in elite paddlers Effect of Omega-3 supplementation on immune responses in elite paddlers 2014-12-22 anticipated 22 Complete https://irct.ir/trial/16946 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Used, Assignment: Parallel, Purpose: Basic scienece. 2 It has not ICD10 code.. Intervention 1: Test group: subjects in test group will recievel 6.0 g/day fish-oil supplementation in capsules (American,Vita Cost) containing 3.6 g of n-3 polyunsaturate fatty acids containing 2400 mg of EPA and 1200 mg DHA for 4 weeks. Moreover, They will experience the increasing intensive exercise during the same time.Blood samples will be collected 48hr before and after study period. Intervention 2: Placebo group: Subjects in placebo group will receive 6gr/day placebo capsules containing mineral oil purchased from Zahravi Pharmaceutical Inc. (Iran) for 4 weeks. Moreover, all subjects will experience the increasing intensive exercise. Blood samples will be collected 48hr before and after study period. http://www.sciencedirect.com/science/article/pii/S0952327815001052 Background<br /> <br /> Physical exercise can induce imbalance of different cytokines by leading them towards an inflammatory and immunosuppressive milieu. Fish-oil (FO) supplementation may modulate the mentioned skewed balance following intense exercise. Therefore, we decided to investigate the effect of intense physical exercise and FO supplementation on cytokine production and helper T (Th) cell phenotype in male elite paddlers.<br /> Subjects and methods<br /> <br /> Male elite paddlers consumed 6 g/day of either FO capsules (n=11) containing 3.6 g long chain n-3 polyunsaturated fatty acids (1.2 g docosahexaenoic acid and 2.4 g eicosapentaenoic acid) or placebo capsules (n=11) for 4 weeks. The paddlers simultaneously undertook a program of increasing exercise. Blood samples were taken from all the subjects 48 h before and after the 4 weeks of supplementation.<br /> Results<br /> <br /> Our results show that while FO supplementation decreases the production of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the elite paddlers, it increases the production of IL-6. On the other hand, while there was no change in IL-4 secretion, the production of interferon (IFN)-γ was significantly decreased after 4 weeks FO consumption. We also showed that the production of IL-10 was significantly higher in the FO group compared to the placebo. Finally, we found that fish-oil consumption shifts the balance between Th cells towards Th2 phenotype during intensive exercise.<br /> Conclusion<br /> <br /> Our results suggest that the consumption of n-3 polyunsaturated fatty acids during intense exercise can induce the anti-inflammatory and immunosuppressive cytokine networks that are associated with a reduced Th1/Th2 ratio in elite paddlers.
public Farhad Jadidi-Niaragh
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Keshavarz boulevard, Tehran, PO Box 6446-14155, Iran
Tehran Iran (Islamic Republic of) +98 21 8895 4913 f-jadidin@razi.tums.ac.ir; farhad_jadidi1986@yahoo.com Tehran University of Medical Sciences scientific Farhad Jadidi-Niaragh
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Keshavarz boulevard, Tehran, PO Box 6446-14155, Iran
Tehran Iran (Islamic Republic of) +98 21 8895 4913 f-jadidin@razi.tums.ac.ir; farhad_jadidi1986@yahoo.com Tehran University of Medical Sciences Iran (Islamic Republic of) The main inclusion criteria: 18 to 30 years of age, non-smoking, agree to avoid sea foods throughout the study,sign informed consent. The main exclusion criteria: History of cardiovascular disease or diabetes, allergy to fish oil, appearance of intestinal side effects such as chronic and acute abdominal pains, diarrhea, and vomiting, acute fever and headache, consumption of anti-coagulation drugs such as aspirin or warfarin. 18 years 30 years Male Other N/A Test group: subjects in test group will recievel 6.0 g/day fish-oil supplementation in capsules (American,Vita Cost) containing 3.6 g of n-3 polyunsaturate fatty acids containing 2400 mg of EPA and 1200 mg DHA for 4 weeks. Moreover, They will experience the increasing intensive exercise during the same time.Blood samples will be collected 48hr before and after study period. Placebo group: Subjects in placebo group will receive 6gr/day placebo capsules containing mineral oil purchased from Zahravi Pharmaceutical Inc. (Iran) for 4 weeks. Moreover, all subjects will experience the increasing intensive exercise. Blood samples will be collected 48hr before and after study period. Age. Timepoint: prior to study. Method of measurement: Questionnaire. Height. Timepoint: prior to study. Method of measurement: meter. Weight. Timepoint: prior to study. Method of measurement: balance. Lean body mass. Timepoint: prior to study. Method of measurement: InBody-520 instrument. Blood fat. Timepoint: prior to study. Method of measurement: laboratory. Maximal oxygen uptake. Timepoint: prior to study. Method of measurement: CASMED instrument. TNF-alpha level. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. IL-1beta. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. IL-6 level. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. IFN-gamma level. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. IL-4 level. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. IL-10 level. Timepoint: 48 hr before and after study period. Method of measurement: ELISA. Th1 frequency. Timepoint: 48 hr before and after study period. Method of measurement: Flow cytometry. Th2 frequency. Timepoint: 48 hr before and after study period. Method of measurement: Flow cytometry. Vice chancellor for research, Tehran University of Medical Sciences Approved 2014-10-28 Ethical Committee of Tehran University of Medical Sciences Ethical Committee of Tehran University of Medical Sciences, Keshavarz boulevard, Tehran, PO Box 6446-14155, Iran Tehran Iran (Islamic Republic of)