IRCT2014020516491N1 IRCT 2014-06-09 Deputy of research, Tabriz University of Medical Sciences The effect of CLA in patients with Non-alcohlic fatty liver disease. The effect of conjugated linoleic acid(CLA) on insulin resitsance, lipid profile, oxidative stress, inflammatory factors and resistin in obese patients with Non-alcoholic fatty liver disease (NAFLD) under weight loss diet :A randomized clinical control trial 2014-02-09 anticipated 46 Complete https://irct.ir/trial/15422 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment. N/A non- alcoholic fatty liver disease. Intervention 1: 3 caposouls of CLA(each containing 1000mg, daily 3000mg) with weight loss diet for eight weeks in intervention group. Intervention 2: weight loss diet for eight weks in control group.

public Dr. Mehrangiz Ebrahimimamagani

Health and Nutrition Faculty,Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street

Tabriz Iran (Islamic Republic of) +98 41 1335 7581 ebrahimimamagani@tbzmed.ac.ir Deputy of Research, Nutrition faculty, Tabriz University of Medical Sciences scientific Haleh Jamali Laleh

Health and Nutrition Faculty,Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street

Tabriz Iran (Islamic Republic of) +98 41 1334 1113 hjl.haleh7@gmail.com Nutrition faculty, Tabriz University of Medical Sciences Iran (Islamic Republic of) Inclusion criteria: Verification of having NAFLD (based on liver ultrasound and liver enzyme levels above normal values based on kits used); 20-50 years old; BMI=30-40; takeing 400IU vitamin E daily. Exclusion criteria: Consumption of alcohol; Pregnancy or lactation and menopause; being athleteic; Inflammatory conditions such as infection; history or perceive of hypertension; Family history of hyperlipidemia; history or perceive of cardiovascular disease; history or perceive of lung disease; history or perceive of renal disease; Liver transplantation; Acute or chronic disorders and other diseases of the liver (hepatitis B, C and liver infections, etc.); Biliary disease; Known Autoimmune disease; Cancer; An inherited disorder affecting the liver status (iron and copper storage disease, etc.); Perceive diseases such as burns and injury during the study; surgery in last 3 months; the use of some medications such as antihypertensitives, insulin sensitivity enhancer, hepatotoxic drugs , statines; Taking any vitamins, minerals and antioxidant supplementation in the last two months; Taking contraceptive pills and estrogen; Consumption of flaxseed; consumption of nuts more than 3 per day . 20 years 50 years Both K76.0 Fatty (change of) liver, not elsewhere classified Treatment - Drugs Treatment - Other 3 caposouls of CLA(each containing 1000mg, daily 3000mg) with weight loss diet for eight weeks in intervention group weight loss diet for eight weks in control group Resistin. Timepoint: Beginning and end of the intervention. Method of measurement: Elisa. Inflammatory factors(IL-6,Il-10,TNFa,hs-CRP). Timepoint: Begining and end of the study. Method of measurement: IL-6,Il-10,TNFa: Elisa and hs-CRP : immunoturbidimetry. Insulin resistance and Insulin sensitivity. Timepoint: Beginning and end of the intervention. Method of measurement: Insulin resistance with HOMA-IR index and Insulin sensitivity with Quicki index. Liver enzymes(AST-ALT-ALP). Timepoint: Beginning and end of the intervention. Method of measurement: IFCC. Lipid profile. Timepoint: Beginning and end of the intervention. Method of measurement: Spectrophotometry and Friedewald Formula for LDL. Oxidative Stress Indicies(TAC-ARE-MDA). Timepoint: Beginning and end of the intervention. Method of measurement: TAC and ARE : spectrophotometry and MDA : thiobarbituric acid. ُSonography faindings. Timepoint: Beginning and end of the intervention. Method of measurement: Liver Sonography. Anthropometry(BMI-WC-WHR-WHtR). Timepoint: Beginning, second, fourth, sixth and end of the intervention. Method of measurement: metr, scale and stadiometr. Body composition. Timepoint: Beginning and end of the intervention. Method of measurement: BIA. Energy and nutrient intake. Timepoint: beginning, fourth week and end of the intervention. Method of measurement: 3 day food record. Comparison of physical activity. Timepoint: beginning, fourth week and end of the intervention. Method of measurement: Physical Activity Questionnaire. Deputy of research, Tabriz University of Medical Sciences Approved 2014-03-03 The Research Ethics Committee of Tabriz University of Medical Sciences Tabriz University of Medical Sciences, Attar Neyshabori Street -Golgasht Street Tabriz Iran (Islamic Republic of)