IRCT2013112515536N1 IRCT 2014-05-14 Tehran University of Medical Sciences The effect of quercetin on PCOS patients Effect of supplementation of quercetin on inflammatory factor and adiponectin and gene expression of its receptors in women’s PBMC with polycystic ovary syndrome(PCOS) 2014-01-21 anticipated 42 Complete https://irct.ir/trial/14782 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Used, Assignment: Parallel, Purpose: Treatment. N/A Polycystic Ovary Syndrome. Intervention 1: to the intervention group Quercetin supplements , 1 g daily for 12 weeks as the two tablets of 500 mg after 2 main meals (breakfast and lunch)and should not be taken on an empty stomach. Intervention 2: Patients in the placebo group will receive 12 weeks of placebo quercetin.

public Neda Rezvan

The fourth floor (Department of Nutrition and Biochemistry)-School of Public Health,Tehran Universit of Medical Sciences, Poursina St-Qods St-Enghelab St-tehran

Tehran Iran (Islamic Republic of) +98 21 8897 4463 neda.rezvan@yahoo.com Tehran University of Medical Sciences scientific Dr. Mohammad Javad Hosseinzadeh-Attar

Faculty of Health-Tehran University of Medical Sciences-Poursina St-ghods st-keshavarz blv-tehran

Tehran Iran (Islamic Republic of) +98 21 8895 1395 hosseinzadeh.mdphd@yahoo.com Tehran University of Medical Sciences Iran (Islamic Republic of) Inclusion criteria: BMI 25 to 40 - the age range of 20-40 years - according to the Rotterdam conference, at least two of the three criteria listed as having the disease. Features include menstrual irregularities, or clinical symptoms and the presence of polycystic ovaries on ultrasound – Hyperandrogenism. donot use drugs; contraceptive drugs, antihypertensive drugs lower blood lipid and anti-inflammatory drug- and willing to cooperate. Exclusion criteria: Endocrine and metabolic diseases such as hypothyroidism and hyperthyroidism, androgen producing cancer, diabetes mellitus, adrenal hyperplasia and Cushing, ovarian tumor and Hyperprolactinaemia - Smoking - Pregnancy - allergic reaction to supplements - Cancel patient to cooperate in the study. 20 years 40 years Female E28.2 Sclerocystic ovary syndrome Stein-Leventhal syndrome Treatment - Drugs Placebo to the intervention group Quercetin supplements , 1 g daily for 12 weeks as the two tablets of 500 mg after 2 main meals (breakfast and lunch)and should not be taken on an empty stomach. Patients in the placebo group will receive 12 weeks of placebo quercetin Serum adiponectin. Timepoint: Before and after intervention. Method of measurement: Adiponectin ELISA Kit μg / ml. Tumor necrosis factor. Timepoint: Before and after intervention. Method of measurement: Serum concentrations of TNFα ELISA kit. C-reactive protein. Timepoint: Before and after intervention. Method of measurement: Serum levels of hs-CRP ELISA Kit. High-molecular weight adiponectin levels. Timepoint: Before and after intervention. Method of measurement: Adiponectin ELISA Kit μg / ml. Glucose. Timepoint: Before and after intervention. Method of measurement: Serum ELISA Kit. Insulin. Timepoint: Before and after intervention. Method of measurement: Serum ELISA kit micro mol / l. Adenosine monophosphate-activated protein kinase. Timepoint: Before and after intervention. Method of measurement: Serum ELISA Kit. Adiponectin receptor 1. Timepoint: Before and after intervention. Method of measurement: RT-PCR. Adiponectin receptor 2. Timepoint: Before and after intervention. Method of measurement: RT-PCR. Testosterone. Timepoint: Before and after intervention. Method of measurement: Serum Elisa kit. Progesterone. Timepoint: Before and after intervention. Method of measurement: serum Elisa kit. Luteinizing hormone. Timepoint: Before and after intervention. Method of measurement: ELISA kit. Follicle stimulating hormone. Timepoint: Before and after intervention. Method of measurement: ELISA kit. Sex Hormone Binding Globulin. Timepoint: Before and after intervention. Method of measurement: IRMA. Systolic blood pressure. Timepoint: Before and after intervention. Method of measurement: Pressure set. Diastolic blood pressure. Timepoint: Before and after intervention. Method of measurement: Pressure set. Total cholesterol. Timepoint: Before and after intervention. Method of measurement: Serum total cholesterol concentration turbidometry mg / dl. Triglyceride. Timepoint: Before and after intervention. Method of measurement: Serum triglyceride concentrations were enzymatically mg / dl. Low-density lipoprotein. Timepoint: Before and after intervention. Method of measurement: LDL-C levels. High-density lipoprotein. Timepoint: Before and after intervention. Method of measurement: Serum HDL cholesterol concentration. Tehran University of Medical Sciences Tehran University of Medical Sciences Tehran University of Medical Sciences Approved 2014-02-15 Ethics Committee of Tehran University of Medical Sciences Sixth Floor-Central Tehran University-Qods St., Keshavarz Blvd-tehran Tehran Iran (Islamic Republic of)