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IRCT2013030912758N1 IRCT 2013-03-25 Iraqi Board for Medical specializations Investigation for chronic Idiopathic Urticaria Autologous Serum Skin Test as a Diagnostic Aid in Chronic Idiopathic Urticaria 2009-11-01 anticipated 54 Complete https://irct.ir/trial/12756 interventional Randomization: Not randomized, Blinding: Not blinded, Placebo: Used, Assignment: Parallel, Purpose: Diagnostic. N/A urticaria. Intervention 1: The Autologous Serum Skin Test (ASST) was performed by drawing 2cubic centimeters of venous blood and stored in a plane tube(not heparinized) and allowed to clot at room temperature ,after that the serum is separated by centrifuging the blood in a centrifuge for 10 minutes at 2000 RPM (Revolution per Minute). 0.05 ml of the patient’s serum is injected intradermally on volar aspect of the patient’s forearm at site not affected by a wheal in the last 24 hours. Intervention 2: At the same time a control test is done by injecting the same volume of normal saline at least 5 centimeters away from the serum injection site.
public Professor Hayder R. Al-Hamamy
Medical city P.O. Box 61211.
baghdad Iraq 12114 009647901309356 Hayder317@gmail.com Head of Department of Dermatology and Venereology, College of Medicine, University of Baghdad, Medic scientific Dr.Ammar Faisal Hameed
Medical city61106
Baghdad Iraq 12114 009647702475782 amar@uobaghdad.edu.iq Department of Dermatology-Baghdad University Iraq Inclusion criteria: All patients with chronic idiopathic urticaria who have active urticaria at time of presentation and the current attack started at least six week before the test. Exclusion criteria: children younger than 15 years of age and pregnant women were excluded from the study no limit no limit Both L50.1 Idiopathic urticaria Diagnosis Diagnosis The Autologous Serum Skin Test (ASST) was performed by drawing 2cubic centimeters of venous blood and stored in a plane tube(not heparinized) and allowed to clot at room temperature ,after that the serum is separated by centrifuging the blood in a centrifuge for 10 minutes at 2000 RPM (Revolution per Minute). 0.05 ml of the patient’s serum is injected intradermally on volar aspect of the patient’s forearm at site not affected by a wheal in the last 24 hours At the same time a control test is done by injecting the same volume of normal saline at least 5 centimeters away from the serum injection site Wheal and flare. Timepoint: 30 minutes after intradermal injection of 0.05 ml of the patient’s serum into patient’s forearm. Method of measurement: wheal and flare at the tested site of a diameter (measured by taking the mean of the largest two diameters) at least 1.5 millimeters more than the wheal and flare induced at the control site. Wheal and flare. Timepoint: 45 minutes after intradermal injection of the patient’s serum into patient’s forearm. Method of measurement: wheal and flare at the tested site of a diameter (measured by taking the mean of the largest two diameters) at least 1.5 millimeters more than the wheal and flare induced at the control site. Iraqi Board for Medical specializations Approved 2009-10-09 Iraqi Board for Medical specializations Medical Collection Office, P.O. Box 61080 Postal Code 12114, Baghdad, Iraq. Baghdad Baghdad Iraq