IRCT2014031112307N3 IRCT 2014-04-21 Tehran University of Medical Sciences Controlled trial of comparison of the effect of controlled ovarian stimulation protocols on gene expression of germinal vesicle oocytes in infertile women Controlled trial of comparison of the effect of controlled ovarian stimulation protocols on gene expression of germinal vesicle oocytes in infertile women 2012-09-20 anticipated 50 Complete https://irct.ir/trial/12396 interventional Randomization: Randomized, Blinding: Single blinded, Placebo: Not used, Assignment: Parallel, Purpose: Basic scienece. N/A Contrlled Ovarian Stimulation protocols. Intervention 1: Control group: In the gonadotropin releasing hormone agonist long protocol group, the administration of Buserelin acetate 500 μg/d is started subcutaneously proceeding the IVF cycle from day 21 until pituitary down regulation. The Buserelin dose is reduced to 250 μg/d until the day of human chorionic gonadotropin (hCG) injection when pituitary down regulation was achieved. Ovarian stimulation is started on the 3rd day of the current menstrual cycle by injection of rFSH Follitropin alfa (Gonal F) at a daily dose of 150 to 225 IU/IM. Human chorionic gonadotropin 5000 IU /2/IM is injected about 34–36 hrs before oocytes pickup day. Intervention 2: Intervention group: In the gonadotropin releasing hormone antagonist fixed multi-dose protocol group, Cetrorelix acetate 0.25 mg/day subcutaneously is initiated on the sixth day of the gonadotropin stimulation until the day of hCG injection. Ovarian stimulation is started on the 3rd day of the current menstrual cycle by injection of rFSH Follitropin alfa (Gonal F) at a daily dose of 150 to 225 IU/IM until the day of human chorionic gonadotropin injection. Human chorionic gonadotropin 5000 IU /2/IM is injected about 34–36 hrs before oocytes pickup day.