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IRCT201208139736N1 IRCT 2012-08-21 Faculty of Medicine, Tehran University of Medical science, Tehran, Iran Effects of ischemia and tissue hypoxia in the pathogenesis of ulcerative colitis: a case control study Effects of ischemia and tissue hypoxia in the pathogenesis of ulcerative colitis: a case control study 2012-04-20 anticipated 60 Complete https://irct.ir/trial/10280 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Diagnostic. N/A Ulcerative Colitis. Intervention 1: Control group 1 included 20 people who are referred for screening with all the features of patients group except history of clinical or colonoscopic bowel disease.Endoscopy and colonoscopy will also apply for control group (they had referred for screening) with preparing one sample from colon and another from body of stomach. Systemic arterial blood gas sampling (ABG) is performed from radial artery after Allen testing. More over, Perirectal capillary blood gas analysis using endoscopic ultrasound (EUS) with 100 cc normal saline and 80 mg gentamycine enema immediately before the procedure is performed. Thirty minutes after perirectal capillary blood gas was taken, blood culture was done to rule out bacteremia .Lipid profile,CBC,FBS,EKGG and S/C was done for all of them.Pathological species transferred to department of pathology for measuring HIF1a. Blood gas samples were assessed within 5 minutes in laboratory by pathologist, and results were recorded. Intervention 2: Control group 2:Ten paraffin wax embedded tissue species from mesenteric ischemia and ten paraffin wax embedded tissue species include infectious colitis entered the study.For this group just tissue ischemic factor by special kits will be assessed. Intervention 3: Patients group interventions: Ten patients with Ulceratve Colitis are selected.Patients should be known case of UC and are diagnosed by clinical symptoms, sigmoidoscopy or colonoscopy findings and pathological species on biopsy.patients group will go under colonoscopy and endoscopy. Three samples are taken from the colon; one from ulcerative tissue, one from normal intestinal tissue and the last one from body of the stomach Systemic arterial blood gas sampling (ABG) are performed from radial artery after Allen testing. More over, Perirectal capillary blood gas analysis using endoscopic ultrasound (EUS) with 100 cc normal saline and 80 mg gentamycine enema immediately before the procedure was performed. Thirty minutes after perirectal capillary blood gas was taken, blood culture is done to rule out bacteremia .Pathological species transfer to department of pathology for measuring HIF1a. Lipid profile, CBC,FBS, S/C, EKG will be done for the patients.Blood gas samples شقثassessed within 5 minutes in laboratory by pathologist, and results were recorded.
public Dr Masih Ttajdini
Faculty of Medicine, Tehran University of Medical science
Tehran Iran (Islamic Republic of) +98 21 6643 7147 Drmasih84@yahoo.com Faculty of Medicine, Tehran University of Medical science scientific Dr Seyed Amir Mirbagheri
Faculty of Medicine, Tehran University of Medical science
Tehran Iran (Islamic Republic of) +98 912 146 2041 Drmasih84@yahoo.com Faculty of Medicine, Tehran University of Medical science Iran (Islamic Republic of) We conducted a randomised clinical trial study to assess the role of ischemia in UC. 20 patients with UC, 20 people without any history of bowel disease and ten paraffin wax embedded tissue species from mesenteric ischemia and ten paraffin wax embedded tissue species include infectious colitis entered the study. UC is diagnosed by clinical symptoms, sigmoidoscopy or colonoscopy findings and pathological species on biopsy.1Patients who took NSAIDs, vasodilators, corticosteroids (some studies showed they might affect tissue oxygenation) and patients with past medical history of ischemic heart disease, pulmonary disorders (COPD, asthma), severe anemia and vascular disease ( vasculitis) and Diabetes which can cause tissue hypoxia were excluded. Control group included 10 people who were referred for screening with all the features mentioned above except history of clinical or colonoscopic bowel disease. Both group had the same age, sex and lived in the same height above the sea level. 18 years 65 years Both K51 Ulcerative colitis Diagnosis Diagnosis Diagnosis Control group 1 included 20 people who are referred for screening with all the features of patients group except history of clinical or colonoscopic bowel disease.Endoscopy and colonoscopy will also apply for control group (they had referred for screening) with preparing one sample from colon and another from body of stomach. Systemic arterial blood gas sampling (ABG) is performed from radial artery after Allen testing. More over, Perirectal capillary blood gas analysis using endoscopic ultrasound (EUS) with 100 cc normal saline and 80 mg gentamycine enema immediately before the procedure is performed. Thirty minutes after perirectal capillary blood gas was taken, blood culture was done to rule out bacteremia .Lipid profile,CBC,FBS,EKGG and S/C was done for all of them.Pathological species transferred to department of pathology for measuring HIF1a. Blood gas samples were assessed within 5 minutes in laboratory by pathologist, and results were recorded. Control group 2:Ten paraffin wax embedded tissue species from mesenteric ischemia and ten paraffin wax embedded tissue species include infectious colitis entered the study.For this group just tissue ischemic factor by special kits will be assessed. Patients group interventions: Ten patients with Ulceratve Colitis are selected.Patients should be known case of UC and are diagnosed by clinical symptoms, sigmoidoscopy or colonoscopy findings and pathological species on biopsy.patients group will go under colonoscopy and endoscopy. Three samples are taken from the colon; one from ulcerative tissue, one from normal intestinal tissue and the last one from body of the stomach Systemic arterial blood gas sampling (ABG) are performed from radial artery after Allen testing. More over, Perirectal capillary blood gas analysis using endoscopic ultrasound (EUS) with 100 cc normal saline and 80 mg gentamycine enema immediately before the procedure was performed. Thirty minutes after perirectal capillary blood gas was taken, blood culture is done to rule out bacteremia .Pathological species transfer to department of pathology for measuring HIF1a. Lipid profile, CBC,FBS, S/C, EKG will be done for the patients.Blood gas samples شقثassessed within 5 minutes in laboratory by pathologist, and results were recorded Pericapillary Blood Gas. Timepoint: Before intervention and after intervention. Method of measurement: Laboratory data reports. SystemicArterial Blood Gas. Timepoint: Before intervention and after intervention. Method of measurement: Laboratory data repots. Tissue ischemic factors. Timepoint: Just after sampling. Method of measurement: Specila kits. Faculty of Medicine, Tehran University of Medical science, Tehran, Iran Approved 2012-03-07 Tehran University of Medical science Tehran,Keshavarz Boulevard,pursina Aveniue Tehran Iran (Islamic Republic of)